Lab Test

Test Definition Number : 246
Test Definition Name : STR analysis for identity / engraftment
Lab Name : OHSU Knight Diagnostics Lab
Lab Test Number of THIS Version : 1181
Lab Test Name : Engraftment (Chimerism) Analysis
Status : Active
Expired : No
Updated : 2018-01-28 07:42 EST

General Attributes

Chromosome Band :
HUGO Gene Name :
ICD-Oncology Code
(includes WHO classification diagnoses)
:

Infectious Disease Attributes

Agent Category :
Non-virus Genus and Species :
Virus Family, Genus, and Species :

Lab-specific Attributes

Method : Electrophoresis - Capillary
Polymerase Chain Reaction (PCR)
Specimen Source
(for clinical patient care these sources must be validated)
: Blood - Whole Blood
Bone Marrow Aspirate
Solid Tissue Paraffin-Embedded Formalin-Fixed
ICD-10 Code :
Use : Clinical - CLIA-certified lab
Genetics Category : Identity testing
SNOMED CT :
LOINC :
Comments : Background: In patients who underwent allogeneic hematopoietic stem cell transplantation, engraftment (chimerism) studies are used to evaluate the level of donor versus recipient cells in post-transplant peripheral blood or bone marrow specimens (and/ or specific sorted cell populations). In order to proceed with engraftment analysis, a pre-transplant specimen is obtained from the recipient and from the donor. DNA from each pre-transplant specimen is extracted and stored for future comparison with post-transplant specimens from the recipient. Engraftment studies are then sequentially performed post-transplant to monitor the level of donor and recipient cell chimerism. Donor and recipient cells are distinguished by PCR amplifying highly polymorphic Short Tandem Repeat (STR) markers, and analyzing the resulting products by capillary electrophoresis. The chimerism analysis is also often performed on flow-sorted purified T cells (often CD3+) and myeloid cells (often CD33+). Clinical Utility: Post-transplant chimerism analysis provides valuable information on the kinetics of engraftment, and assists in the diagnosis of graft failure, rejection, and relapse. In certain clinical situations, evaluation of mixed chimerism in lineage-specific cells – for example, flow-sorted CD3+ or CD33+ cells, can provide useful prognostic information for long-term engraftment. Methodology: DNA is extracted from sorted or unsorted cells, PCR amplified with a series of primers to 10 different polymorphic loci, and the PCR amplicons are analyzed by capillary electrophoresis. Engraftment analysis is based on the ABI Profiler Plus human identity test kit.
Specimen Transport Requirements : 5-10mL Blood or Bone Marrow (ACD or EDTA) Sorted Cell Chimerism: 5-10 mL of blood or bone marrow in green (Sodium Heparin) tube (unspun) Package and ship specimens to remain cold, but not frozen. Ship via overnight express. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
Samples Available for Proficiency Test Sample Exchange : Yes

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