Lab Test

Test Definition Number : 163
Test Definition Name : Gene sequencing panel (<=30 genes)
Lab Name : OHSU Knight Diagnostics Lab
Lab Test Number of THIS Version : 1183
Lab Test Name : GeneTrails Colorectal Cancer Mutation Panel
Status : Active
Expired : No
Updated : 2018-01-28 07:46 EST

General Attributes

Chromosome Band :
HUGO Gene Name :
ICD-Oncology Code
(includes WHO classification diagnoses)
:

Infectious Disease Attributes

Agent Category :
Non-virus Genus and Species :
Virus Family, Genus, and Species :

Lab-specific Attributes

Method : Sequencing - Next Generation (Massively Parallel) (NGS)
Specimen Source
(for clinical patient care these sources must be validated)
: Solid Tissue Paraffin-Embedded Formalin-Fixed
ICD-10 Code :
Use : Clinical - CLIA-certified lab
Genetics Category : Acquired (somatic)
SNOMED CT :
LOINC :
Comments : Background: It is well established that EGFR-targeted therapies such as cetuximab and panitumumab are ineffective against colorectal cancers harboring mutations in KRAS codons 12, 13 or 61. Recently, several studies have shown that other, more rare mutations in KRAS, as well as mutations in NRAS, also predict for resistance to these drugs. In addition, multiple studies have shown that BRAF mutation V600E correlates with a significantly worse prognosis and lack of response to cetuximab and panitumumab. Methodology: 1. Microscopic examination of the specimen and macrodissection of tumor-rich areas 2. DNA extraction and purification 3. Next-generation DNA sequencing Estimated sensitivity: at least 15% mutant allele; estimated specificity >99%
Specimen Transport Requirements : Paraffin block or 10 unstained sections (4-5 micron) Due to DNA damage, decalcified specimens are not recommended. Package and ship samples at room temperature. Ship via overnight express. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
Samples Available for Proficiency Test Sample Exchange : Yes

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