Lab Test

Test Definition Number : 163
Test Definition Name : Gene sequencing panel (<=30 genes)
Lab Name : OHSU Knight Diagnostics Lab
Lab Test Number of THIS Version : 1184
Lab Test Name : GeneTrails GIST Genotyping Panel
Status : Active
Expired : No
Updated : 2018-01-28 07:45 EST

General Attributes

Chromosome Band :
HUGO Gene Name :
ICD-Oncology Code
(includes WHO classification diagnoses)
:

Infectious Disease Attributes

Agent Category :
Non-virus Genus and Species :
Virus Family, Genus, and Species :

Lab-specific Attributes

Method : Sequencing - Next Generation (Massively Parallel) (NGS)
Specimen Source
(for clinical patient care these sources must be validated)
: Solid Tissue Paraffin-Embedded Formalin-Fixed
ICD-10 Code :
Use : Clinical - CLIA-certified lab
Genetics Category : Acquired (somatic)
SNOMED CT :
LOINC :
Comments : Background: Screening for mutations in oncogenes and tumor suppressor genes is increasingly important in delivering personalized cancer care. The GeneTrails GIST Panel delivers information on a variety of treatment-informative mutations in 23 genes commonly involved in GI stromal tumors 1-9, covering all of the hotspot mutations sites in the genes listed below. The panel as a sensitivity of <=10% mutant allele. Genes included in panel: AKT1, BRAF, KRAS, PDGFRA, SDHA, SDHC, AKT2, CDKN2A, MAP2K1, PIK3CA, SDHAF1, SDHD, AKT3, HRAS, NF1, PTEN, SDHAF2, TP53, ATM, KIT, NRAS. PTPN11, SDHB Methodology: The GIST Panel is performed on DNA from formalin-fixed, paraffin-embedded (FFPE) tumor tissue using next-generation, semiconductor-based sequencing (Ion Torrent platform). Input DNA is amplified using the AmpliSeq technology (Ion Torrent), after which the amplicons are modified with adaptors, re-amplified, and subjected to emulsion PCR. The final products are sequenced on a semiconductor-based chip.
Specimen Transport Requirements : Paraffin block or 10 unstained sections (4-5 micron) Due to DNA damage, decalcified specimens are not recommended. Package and ship samples at room temperature. Ship via overnight express. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
Samples Available for Proficiency Test Sample Exchange : Yes

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