Lab Test

Test Definition Number : 163
Test Definition Name : Gene sequencing panel (<=30 genes)
Lab Name : OHSU Knight Diagnostics Lab
Lab Test Number of THIS Version : 1185
Lab Test Name : GeneTrails NSCLC Genotyping Panel
Status : Active
Expired : No
Updated : 2018-01-28 07:45 EST

General Attributes

Chromosome Band :
HUGO Gene Name :
ICD-Oncology Code
(includes WHO classification diagnoses)
:

Infectious Disease Attributes

Agent Category :
Non-virus Genus and Species :
Virus Family, Genus, and Species :

Lab-specific Attributes

Method : Sequencing - Next Generation (Massively Parallel) (NGS)
Specimen Source
(for clinical patient care these sources must be validated)
: Solid Tissue Paraffin-Embedded Formalin-Fixed
ICD-10 Code :
Use : Clinical - CLIA-certified lab
Genetics Category : Acquired (somatic)
SNOMED CT :
LOINC :
Comments : Background: Screening for mutations in oncogenes and tumor suppressor genes is increasingly important in delivering personalized cancer care. The GeneTrails NSCLC Panel delivers information on a variety of treatment-informative mutations in the 23 genes listed below, which are known to play a role in cases of non-small cell lung cancer. The next-gen sequencing panel has a sensitivity of <=10% mutant allele. Genes included in this panel: AKT1, ALK, BRAF, CDKN2A, DDR2, EGFR, ERBB2, HRAS, JAK2, KDR, KRAS, MAP2K1, NOTCH1, NRAS, NTRK1, NTRK2, NTRK3, PIK3CA, PIK3R1, PIK3R2, PTEN, PTPRD, TP53 In addition to the sequencing panel, tumor samples can be screened by FISH for translocations involving the ALK, ROS1 or RET kinase genes, and for MET gene amplification. This FISH panel may be added to the sequencing panel by ordering both test codes 5120 and 7600. The FISH probes may be ordered individually as well: ALK (test code 8019), ROS1 (test code 8720), RET (test code 8700) and MET (test code 8500). Recommended Use: The GeneTrails NSCLC Panel is well-suited for screening potentially actionable mutations in the non-small cell lung carcinomas, including adenocarcinomas, squamous cell carcinomas, and large cell undifferentiated carcinomas. Methodology: The GeneTrails NSCLC Panel is performed on DNA from formalin-fixed, paraffin-embedded (FFPE) tumor tissue using next-generation, semiconductor-based sequencing (Ion Torrent platform). Input DNA is amplified using the AmpliSeq technology (Ion Torrent), after which the amplicons are modified with adaptors, re-amplified, and subjected to emulsion PCR. The final products are sequenced on a semiconductor-based chip.
Specimen Transport Requirements : Paraffin block or 10 unstained sections (4-5 micron) Due to DNA damage, decalcified specimens are not recommended. Package and ship samples at room temperature. Ship via overnight express. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
Samples Available for Proficiency Test Sample Exchange : Yes

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