Lab Test

Test Definition Number : 163
Test Definition Name : Gene sequencing panel (<=30 genes)
Lab Name : OHSU Knight Diagnostics Lab
Lab Test Number of THIS Version : 1186
Lab Test Name : GeneTrails Solid Tumor Fusion Gene Panel
Status : Active
Expired : No
Updated : 2018-01-28 07:44 EST

General Attributes

Chromosome Band :
HUGO Gene Name :
ICD-Oncology Code
(includes WHO classification diagnoses)

Infectious Disease Attributes

Agent Category :
Non-virus Genus and Species :
Virus Family, Genus, and Species :

Lab-specific Attributes

Method : Sequencing - Next Generation (Massively Parallel) (NGS)
Specimen Source
(for clinical patient care these sources must be validated)
: Solid Tissue Paraffin-Embedded Formalin-Fixed
ICD-10 Code :
Use : Clinical - CLIA-certified lab
Genetics Category : Acquired (somatic)
Comments : Background: In recent years an increasing number of gene fusions have been identified in solid tumors, many of them involving kinases that are targetable with specific therapies. Examples include fusions involving ALK (e.g. EML4-ALK) and ROS1 in non-small cell carcinoma, RET in thyroid carcinoma, BRAF in some types of glioma, and FGFR2 in cholangiocarcinoma.1-6 The GeneTrails Solid Tumor Fusion Gene panel is designed to detect fusions involving the 20 target genes listed below. The panel is performed using next-generation sequencing of tumor RNA, and is useful for finding clinically actionable fusions that are not routinely screened by FISH. Control genes are included on the panel to assess specimen adequacy. The assay has high sensitivity (to the level of approximately 1%, or 1 positive cell in 100). Fusion Genes: AKT3, ALK, BRAF, CD74, EGFR, EML4, ERBB4, ERG, ETV6, FGFR1, FGFR2, FGFR3, MET, NOTCH1, NOTCH2, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, PDGFRA, RAF1, RET, ROS1 Housekeeping Genes: HMBS, ITGB7, LMNA, MYC, TBP Methodology: Microscopic examination of the specimen and macrodissection of tumor-rich areas Nucleic acid extraction and purification Next-generation RNA sequencing Estimated sensitivity: at least 15% mutant allele; estimated specificity >99%
Specimen Transport Requirements : Paraffin block or 10 unstained sections (4-5 micron) Due to DNA damage, decalcified specimens are not recommended. Package and ship samples at room temperature. Ship via overnight express. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
Samples Available for Proficiency Test Sample Exchange : Yes


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