Lab Test

Test Definition Number : 199
Test Definition Name : PML-RARA t(15;17) translocation detection
Lab Name : OHSU Knight Diagnostics Lab
Lab Test Number of THIS Version : 1239
Lab Test Name : PML-RARA Quantitative RT-PCR
Status : Active
Expired : No
Updated : 2018-05-04 09:25 EDT

General Attributes

Chromosome Band : 15q24.1
HUGO Gene Name : [PML] promyelocytic leukemia
[RARA] retinoic acid receptor, alpha
ICD-Oncology Code
(includes WHO classification diagnoses)

Infectious Disease Attributes

Agent Category :
Non-virus Genus and Species :
Virus Family, Genus, and Species :

Lab-specific Attributes

Method : Polymerase Chain Reaction (PCR) - Real Time
Specimen Source
(for clinical patient care these sources must be validated)
: Blood - Whole Blood
Bone Marrow Aspirate
ICD-10 Code :
Use : Clinical - CLIA-certified lab
Genetics Category : Acquired (somatic)
Comments : Acute promyelocytic leukemia (APL) accounts for 5% to 10% of acute myeloid leukemia, and generally has a good prognosis with current treatment protocols. APL cells contain a characteristic translocation fusion gene comprised of the downstream sequences of the retinoic acid receptor alpha gene (RARA) fused to the promoter region and upstream sequences of the promyelocytic leukemia gene (PML). The fusion gene is designated PML/RARA and is the consequence of the t(15;17)(q22;q12) translocation. Messenger RNA (PML/RARA) produced from the fusion gene can be quantitatively detected using a PCR-based assay, and indicates the presence and relative amount of leukemia cells. The PCR-based assay has greater sensitivity than standard methods such as morphology, karyotyping, or FISH.
Specimen Transport Requirements : 5-10mL Blood or Bone Marrow (ACD or EDTA) Package and ship specimens to remain cold, but not frozen. Ship via overnight express. Contact Client Services at (855) 535-1522 for shipping kits and instructions.
Samples Available for Proficiency Test Sample Exchange : Yes


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